Evaluation of a hexane/isopropanol lipid solvent system for analysis of bacterial phospholipids and application to chloroform-soluble Nuclepore (polycarbonate) membranes with retained bacteria
نویسندگان
چکیده
The recovery of phospholipid, ester-linked fatty acids and phospholipid phosphate from the eubacterium Pseudomonas atlantica was shown to be equivalent for the modified Bligh and Dyer (chloroform/methanol/water) procedure and a hexane/isopropanol (HIP) lipid extraction. In addition to its previously suggested lower toxicity, the HIP solvent system allows lipid analyses to be routinely done on chloroform-soluble materials such as Nuclepore polycarbonate membranes, useful for well-defined size fractionation studies of bacterioplankton communities.
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